Fusogenic Liposomes Encapsulating Functionalized

**Fusogenic liposomes encapsulating functionalized magnetic nanoparticles for a direct access to the cytosol.**:

- Réf **ABG-126825**
- Stage master 2 / Ingénieur- Durée 5 mois- Salaire net mensuel 630€- 12/11/2024- Sorbonne Université - laboratoire PHENIX- Lieu de travail- Paris Ile-de-France France- Champs scientifiques- Chimie
- Mots clés- fusogenic liposomes, magnetic nanoparticles, magnetic hyperthermia, cancer treatment-
- 30/11/2024**Établissement recruteur**:
**Site web**:
Sorbonne University is a world-class, research-intensive university bringing together a broad range of arts, humanities, social sciences, natural sciences, engineering and medicine. The scientific Pierre and Marie Curie campus was completely refurbished in 2016. PHENIX is a laboratory at the interface between Chemistry, Physics and Materials Science with a long-standing expertise of colloidal systems, electrolytes and fluids under confinement. Its strength lies in a combination of experimental and modelling activities (numerical simulations). Several international projects and networks are in place in PHENIX, providing a rich and multinational environment. This internship will take place in the "Colloïdes INorganiques" team.

**Description**:
1. Description of the project

In the PHENIX laboratory, we have been studying several strategies for MNP to avoid being trapped inside endosomes. The use of fusogenic liposomes, liposomes that are able to fuse with the cellular membrane to deliver its content directly inside the cytosol[5], is one of them. The goal of this M2 internship will be to study the encapsulation of core-shell γ-Fe2O3-SiO2 nanoparticles inside fusogenic liposomes. The core-shell MNP will be synthesized so that they can diffuse in the cytosol (negatively charged, diameter smaller than 75 nm) and functionalized with peptides or antibodies in order to target intracellular mitochondria. Then the formation of fusogenic liposomes encapsulating these MNP will be thoroughly investigated, and the hybrid nano-objects will be fully characterized. The capacity of the liposomes to deliver the MNP into the cell cytosol will then be studied by fluorescence microscopy. Finally, their efficiency in killing cancer cells under an alternating magnetic field through magnetic hyperthermia will be determined.

2. Specific techniques or methods

Liposomes synthesis by reverse phase evaporation.

Synthesis of magnetic nanoparticles made of an iron oxide core and a silica shell by sol-gel chemistry.

Bio-functionalization of MNP.

Dynamic light scattering, zetametry, transmission electron microscopy

Cell culture, fluorescence and confocal microscopy.

Magnetic hyperthermia.
- 3. _References_
- [1] Cardoso, V.F. et al., Adv. Healthc. Mater. 7, 1700845 (2018); [2] Beik, J. et al., J. Controlled Release 235, 205-221 (2016);[3] Monzel, C. et al., Chem. Sci. 8, 7330-7338 (2017) [4] Di Corato, R. et al., Biomaterials 35 (24), 6400-6411 (2014); [5] Chen, F. et al., RSC Adv. 2021, 11 (57), 35796-35805.**Profil**:
We are looking for a highly motivated chemist, really interested by research at the interface between chemistry, nanomaterials and biology.

**Prise de fonction**:

- 27/01/2025