Research Associate
il y a 3 semaines
A lab-based position, your work will focus on the engineering of mammalian cell lines and analysis of therapeutic proteins produced in engineered cell lines.
**Roles and responsibilities**
- Generate stable mammalian cell lines expressing therapeutic proteins of interest
- Optimise expression of genes of interest in mammalian cells by testing different promoters, UTR’s, etc.
- Purify recombinant proteins produced in mammalian cells and prepare high-quality samples for downstream analysis
- Characterize purified therapeutic proteins:
- Quantification
- Western blotting
- Activity assays
- Stability assays
- Protein secondary structure determination
- Glycoprofiling using mass spectrometry (sample preparation, execution, and analysis)
- Be an active part of a fast-growing R&D team
**Description profil**:
PhD in glycobiology, cell and molecular biology, biochemistry, biological engineering or related field
Experience working with glycoproteins and/or the protein secretory pathway
Excellent basic molecular biology techniques: mammalian cell culture, transfections, bacterial cloning, PCR and qPCR, fluorescence microscopy and protein analysis
At least one first author academic paper or equivalent proof of scientific leadership
Mass spectrometry experience
Someone who wants to push the boundaries of scientific knowledge and tackle things they have never done before (or no one has)
Fluency in English
A team player who is strongly invested in our mission at kyron.bio and willing to go the extra mile
**Bonus skills**:
- Postdoctoral studies
- Experience in a biotech/pharmaceutical company
- Prior expertise with CHO or HEK cell lines
- Patent inventor
- Experience working with plant and yeast recombinant expression systems
- Strong bioinformatic skills
- French language
**Poste proposé**:
**Intitulé du poste proposé**
Kyron.bio - Research Associate - Glycobiology Specialist
**Poste basé à **Paris
**Secteur d’activité **:Biotechnology
**Nature du contrat **:CDI
**Disponibilité **:A partir de août 2023
**Description entreprise**:
**kyron.bio** has made giant leaps in drug discovery over the past 5 years, however the bioproduction industry cannot keep up. kyron.bio is an early stage start-up with a mission to revolutionise therapeutic protein production. Our Next Generation Bioproduction Platform will boost both the effectiveness and the yield of proteins produced in mammalian cells. We are doing this by harnessing highly efficient processes from diverse organisms to genetically engineer the drug production platform of the future. We are the first company to use this approach and have a patent pending.
Our vision is to generate the drug production platform of the future that will allow life-saving treatments to reach those that are most in need. We would love for you to join us on this mission.
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