Stage de Master 2 en Biologie

**Intitulé du poste**:
Stage de Master 2 en biologie H/F

**Type de contrat**:
STAGE

**Statut**:
Non cadre

**Disponibilité du poste**:
01/02/2025

**Localisation du poste**:
Fontenay-aux-Roses

**Environnement / Organisation / Contexte**:
Stage de Master 2 en biologie au sein du LRMED

**Mission**:
Endothelial-immune crosstalk in the lung after exposure to ionizing radiation
The immune system is essential for tumor control and plays a crucial role in the response of normal tissues to radiotherapy.1 The vascular endothelium prevents the passage of immune cells from the bloodstream to tissues under normal conditions and organizes the recruitment of immune cells during stress, injury, or infection. Irradiation makes endothelial cells more adherent to circulating immune cells, activates transendothelial migration, and promotes immune infiltration. Modified in all their functions, endothelial cells are key actors in the response of tumors to irradiation and in the initiation of radiotherapy-induced sequelae.
We study the molecular changes in endothelial cells caused by ionizing radiation, focusing on their behavior with different irradiation modalities to develop tools predicting radiation-induced sequelae risks. We have focused on the mechanisms underlying immune cell recruitment by irradiated endothelial cells, using multi-omics and real-time microscopy imaging to highlight the role of glycosylation of endothelial surface proteins in interactions with monocytes.2 Single cell transcriptome analysis from mouse lungs irradiated with a fibrosing dose are investigating how endothelial and immune cells behave and interact in vivo under conventional irradiation and different modalities such as ultra-high dose rates.
A scRNA-seq dataset from irradiated mouse lungs was generated to study lung radiation-induced fibrosis, involving 24 mice at various post-irradiation times. Endothelial and immune cells were isolated and sequenced, resulting in 48 samples and approximately 375,000 cells analyzed. We are exploring potential interactions between immune and endothelial cells across different cell types. Additional experiments compare transcriptomic responses at the single-cell level under another irradiation modality. Future experiments will study the flash effect on lung endothelial and immune cell recruitment at ultra-high dose rates compared to conventional irradiation. For this Master internship, the student will be involved in analyzing single-cell transcriptomic data and carrying out scRNA-seq experiments based on whole-thorax irradiation to propose hypotheses on endothelial cell response mechanisms and interactions with immune cells. These hypotheses will be explored in vitro using gene expression interference with various tools in place in the laboratory, including real-time imaging and videomicroscopy.

**Profil recherché**:
en fin de master2, profil universitaire ou école d'ingénieur

**Télétravail**:
Non

**Diversité**:
**La diversité est une des composantes de la politique RSE, RH et Qualité de Vie au Travail à l’IRSN.**

**L'IRSN conduit une politique active depuis de nombreuses années en faveur de l'égalité des chances au travail et l'emploi des personnes handicapées. Si vous êtes en situation de handicap, n'hésitez pas à nous faire part de vos éventuels besoins spécifiques afin que nous puissions les prendre en compte.**

**Localisation du poste**:
**Localisation du poste**:
Europe, France, Ile-de-France, Hauts-de-Seine (92)

**Langues**:

- Français (3- Niveau avancé)
- Anglais (2- Niveau professionnel)