Application Engineer Position for Nmr Monitoring of

Il y a 2 mois


Nantes, France Nantes University Temps plein

**Site web**:
**Poste et missions**:
**Context**:Oleaginous microalgae are a source of biomass rich in oil (or triacylglycerol, TAG), an energetically dense class of molecules generated by esterification of fatty acids (FAs). FAs are carbon chains of various structures, which can be of interest to different industrial sectors, from very long-chain polyunsaturated GAs for food, health and cosmetics, to monounsaturated/saturated GAs for commodity chemistry and biofuels. Oil from algae is therefore one of the most promising renewable alternatives to fossil hydrocarbons. As part of the B-BEST priority research program and equipment, the diatom Phaeodactylum tricornutum (PT) will be studied, as it is known to produce large quantities of lipids as TAGs droplet form in the extracellular medium.- The CEISAM and GEPEA laboratories involved in the ALGADVANCE project have a 6-year collaboration in which they demonstrated, as part of the AMER-METAL project, that Nuclear Magnetic Resonance (NMR) spectroscopy gives precise access to the identity and quantity of microalgal lipids [1] directly on the whole biomass, thanks to high magnetic field instruments. On the other hand, compact NMR devices [2] were able to monitor the lipid production of microalgae, thanks to signal elimination from the water medium [3] during 15 days of culture by coupling a photobioreactor [4].**References**:

- [1] Dylan Bouillaud, these de doctorat, 2017-2020, Multiscale NMR analysis of the microalgae metabolism
**Mobilité géographique**:

- Nationale**Télétravail**:

- Occasionnel**Prise de fonction**:

- 06/05/2024**Profil **:**Objectifs**:
**Objectives**: The main objective will be to characterize the lipids produced by PT, using a high-field instrument (700 MHz of the CEISAM site in Nantes) to identify the different lipid classes and chains not only in extracts, but also in the challenging whole cells _in vivo_. In this context, we will be implementing methods that are more resolutive, faster and more sensitive, and capable of overcoming intracellular inhomogeneities. In addition, the high repeatability of NMR makes it a tool of choice for quantifying identified lipids in mixtures in cell and in the cultivation medium. Secondly, we will evaluate the performance of a 80 MHz instrument (CEISAM and GEPEA in St Nazaire sites) to validate this device for identifying and quantifying PT lipids, in comparison with information obtained at higher field at 700 MHz. Finally, we will be able to monitor PT cultures in real time by coupling the 80 MHz spectrometer to a photobioreactor as a culture seat. This part will be carried out in collaboration with the PhD student of the project working on the GEPEA site.



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