Research Associate

il y a 2 jours


SoisysurSeine, Île-de-France Kyron Temps plein

About the Role

We are seeking a highly skilled Research Associate to join our team at Kyron, specializing in glycobiology. As a key member of our R&D team, you will be responsible for the engineering of mammalian cell lines and analysis of therapeutic proteins produced in engineered cell lines.

Key Responsibilities

  • Develop and optimize stable mammalian cell lines expressing therapeutic proteins of interest
  • Conduct experiments to optimize gene expression in mammalian cells, including testing different promoters and UTRs
  • Purify recombinant proteins produced in mammalian cells and prepare high-quality samples for downstream analysis
  • Characterize purified therapeutic proteins through various techniques, including quantification, Western blotting, activity assays, stability assays, and protein secondary structure determination
  • Perform glycoprofiling using mass spectrometry, including sample preparation, execution, and analysis
  • Collaborate with the R&D team to advance our research and development goals

Requirements

  • PhD in glycobiology, cell and molecular biology, biochemistry, biological engineering, or a related field
  • Experience working with glycoproteins and/or the protein secretory pathway
  • Excellent basic molecular biology techniques, including mammalian cell culture, transfections, bacterial cloning, PCR, and qPCR
  • Fluency in English
  • Strong team player with a passion for scientific discovery and a willingness to push the boundaries of knowledge

Bonus Skills

  • Postdoctoral studies
  • Experience in a biotech/pharmaceutical company
  • Prior expertise with CHO or HEK cell lines
  • Patent inventor
  • Experience working with plant and yeast recombinant expression systems
  • Strong bioinformatic skills

About Kyron

Kyron is a biotechnology company dedicated to advancing scientific knowledge and developing innovative solutions. We are committed to fostering a collaborative and dynamic work environment that encourages creativity and growth.



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