Postdoctoral fellow position M/F in Biochemistry, Cell Biology et Virology

il y a 1 mois


Montpellier, France CNRS Temps plein

Informations générales

Intitulé de l'offre : Chercheur(e) Postdoctoral(e) H/F en Biochimie, Biologie Cellulaire et Virologie
Référence : UMR9004-DELMUR-004
Nombre de Postes : 1
Lieu de travail : MONTPELLIER
Date de publication : mardi 22 octobre 2024
Type de contrat : CDD Scientifique
Durée du contrat : 12 mois
Date d'embauche prévue : 1 février 2025
Quotité de travail : Temps complet
Rémunération : à partir de 3021.50€ brut mensuel, ajustable selon expérience
Niveau d'études souhaité : Niveau 8 - (Doctorat)
Expérience souhaitée : 1 à 4 années
Section(s) CN : Biologie moléculaire et structurale, biochimie

Missions

L'agent devra étudier l'assemblage du virus RSV (Respiratory Syncitial Virus): en particulier il étudiera les interactions de la protéine M avec les lipides de la cellule et d'autres protéines du virus:
1- in vitro sur des membranes lipidiques biomimétiques (la protéine M et ses mutants seront préparé par le consortium)
2- ex vivo dans des lignées cellulaire par microscopies de fluorescence du vivant ou par imagerie de super-résolution confocale / STED.

Activités

- fabrication de membranes biomimétiques (LUV / SLB)
- etude de l'interaction de la protéine M et de ses mutants avec les LUV
- etude du recrutement des lipides par M et ses mutants sur SLB
- production de plasmides ADN
- culture cellulaire en BSL2, transfection
- etude de la localisation de M et d'autres protéines virales fluorescentes dans des cellules pulmonaires au cours du temps (microscopie du vivant et imagerie par microscopie confocale/STED par immuno-fluorescence)
- recrutement de lipides par le virus dans les cellules de poumon
- presentation des resultats en lab meeting, et en congres nationaux et internationaux
- anglais requis
- esprit d'équipe
- travail en BSL1, BSL2 (culture cellulaire) et BSL3 possiblement
- visite dans les laboratoires du consortium

Compétences

Biochimie des lipides
Culture cellulaire
Microscopie confocale (minimum)
Virologie moleculaire et cellulaire
Biophysique des membranes est un plus
Anglais B1/B2 requis

Contexte de travail

Le/la chercheur(e) postdoctoral(e) travaillera au CNRS de Montpellier à l'IRIM (Institut de Recherche en Infectiologie de Montpellier) dans l'équipe MDVA (Domaines Membranaires et Assemblage Viral). Cette équipe s'intéresse à l'assemblage des virus enveloppés à ARN et le rôle des interactions des protéines virales structurales avec l'ARN viral et les lipides de la cellule hôte par des techniques de virologie, biochimie, biologie cellulaire et microscopie de super-résolution. Cette équipe comprends actuellement 3 chercheurs (en Virologie, biophysique et biologie moléculaire), une ingénieure (en biologie cellulaire), 2 Post-Doctorantes, 4 Doctorant(e)s et une Master. c'est une équipe interdisciplinaire et internationale.

Le poste se situe dans un secteur relevant de la protection du potentiel scientifique et technique (PPST), et nécessite donc, conformément à la réglementation, que votre arrivée soit autorisée par l'autorité compétente du MESR.

Contraintes et risques

Travailler en BSL3

Informations complémentaires

Ce projet de recherche RSV-Mlipo est financé par l'ANR avec un consortium national qui implique l'IRIM à Montpellier, l'IBS de Grenoble et l'INRAE de Jouy-en-Josas (de 2024 à 2027).



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