Stage M2: The role of miRNA in myogenic cell communication in response to ionizing radiation H/F
il y a 1 mois
Mission
Cell-to-cell communication is crucial for tissue homeostasis, enabling it to respond to stress, such as irradiation. MicroRNAs (miRNAs) play a key role in this process by regulating gene expression in target cells.
The main objective of this internship is to validate preliminary data identifying potential candidate miRNAs found in cell culture supernatants after irradiation of myogenic cells. To achieve this, the candidate will improve the characterization of the expression profile of a panel of candidate miRNAs by quantitative PCR on samples of the murine myogenic C2C7 cell line and compare the profiles obtained between the different myogenic differentiation stages, i.e. myoblasts (proliferative cells), myocytes (differentiated cells) and myotubes (differentiated and polynucleated cells) exposed to different doses of irradiation.
At the end of the internship, the candidate could examine the communication impact of the modulation of some of these miRNAs on the different myogenic cells, in terms of cell survival by using MTT assay and activation of DNA damage response by immunostaining.
Profil recherché
This internship will allow the selected student to improve their technical skills in cell biology and molecular biology. In addition, the candidate will acquire solid knowledge in exposure to ionizing radiation, as well as their consequences on eukaryotic cells.
We are seeking for highly motivated Master 2 student in biology / health sciences, with good knowledge in cellular and molecular biology.
Télétravail
Non
Diversité
La diversité est une des composantes de la politique RSE, RH et Qualité de Vie au Travail à lIRSN.
Nous accordons la même considération à toutes les candidatures, sans discrimination, pour inclure tous les talents.
Quelles que soient les différences, nous souhaitons attirer, intégrer et fidéliser nos candidats et nos collaborateurs au sein dun environnement de travail inclusif.
L'IRSN conduit une politique active depuis de nombreuses années en faveur de l'égalité des chances au travail et l'emploi des personnes handicapées. Si vous êtes en situation de handicap, n'hésitez pas à nous faire part de vos éventuels besoins spécifiques afin que nous puissions les prendre en compte.
Localisation du poste
Localisation du poste
Europe, France, Ile-de-France, Hauts-de-Seine
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Nanterre, Île-de-France IRSN Temps pleinJob DescriptionMissionThe primary objective of this project is to elucidate the mechanisms underlying the cellular responses to radiation-induced DNA damage during myogenic differentiation. This involves investigating the impact of ionizing radiation on the DNA repair processes in myotubes, which are polynuclear cells of skeletal muscle tissue, and comparing...
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Nanterre, Île-de-France IRSN Temps pleinProject OverviewThe goal of this project is to investigate how myotubes, a type of polynuclear cell found in skeletal muscle tissue, maintain their cellular functions when their nuclei are damaged due to irradiation. This project aims to understand the complex mechanisms of DNA repair and how they impact the genomic integrity of these cells.Research...
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